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Issue 11, 2014
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Detection of molecular binding via charge-induced mechanical response of optical fibers

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Abstract

We report a charge sensitive optical detection technique for the label-free study of molecular interactions. Traditional label-free optical detection techniques largely rely on the detection of the mass of a molecule, and are insensitive to small molecules. In contrast, the present technique detects the charge of a molecule, where the signal does not diminish with the size of the molecule, thus making it capable for studying small molecules. In addition, the technique is compatible with the standard microplate platform, making it suitable for high-throughput screening of drug candidates. Using this technique, we have detected 0.2 nM anti-BSA and 15 μM anti-cancer drug (imatinib) with an enzyme modified surface. The achieved effective charge detection limit is ∼0.25 electron charge per μm2, corresponding to ∼0.3 fg mm−2 for imatinib, which is orders of magnitude better than the traditional label-free optical detection methods.

Graphical abstract: Detection of molecular binding via charge-induced mechanical response of optical fibers

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Publication details

The article was received on 24 Apr 2014, accepted on 21 Jun 2014 and first published on 03 Jul 2014


Article type: Edge Article
DOI: 10.1039/C4SC01188K
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Chem. Sci., 2014,5, 4375-4381

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    Detection of molecular binding via charge-induced mechanical response of optical fibers

    Y. Guan, X. Shan, S. Wang, P. Zhang and N. Tao, Chem. Sci., 2014, 5, 4375
    DOI: 10.1039/C4SC01188K

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