Jump to main content
Jump to site search

Issue 110, 2014
Previous Article Next Article

Crystal structure, stability and Ago2 affinity of phosphorodithioate-modified RNAs

Author affiliations

Abstract

Small interfering RNAs (siRNAs) with phosphorodithioate modifications (PS2-RNA) possess favourable properties for use as RNAi therapeutics. Beneficial here is the combining of PS2 and 2′-O-methyl modifications (MePS2). SiRNAs with MePS2 moieties in the sense strand show promising efficacies in vitro and in vivo. Crystal structures of PS2- and MePS2- modified RNAs reveal subtle changes in geometry and hydration compared with natural RNA. A model of an MePS2-RNA–PAZ domain complex points to a hydrophobic effect as the source of the higher affinity of MePS2-RNA for Ago2.

Graphical abstract: Crystal structure, stability and Ago2 affinity of phosphorodithioate-modified RNAs

Back to tab navigation

Supplementary files

Article information


Submitted
22 Sep 2014
Accepted
18 Nov 2014
First published
20 Nov 2014

This article is Open Access

RSC Adv., 2014,4, 64901-64904
Article type
Communication
Author version available

Crystal structure, stability and Ago2 affinity of phosphorodithioate-modified RNAs

P. S. Pallan, X. Yang, M. Sierant, N. D. Abeydeera, T. Hassell, C. Martinez, M. Janicka, B. Nawrot and M. Egli, RSC Adv., 2014, 4, 64901
DOI: 10.1039/C4RA10986D

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

Reproduced material should be attributed as follows:

  • For reproduction of material from NJC:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
  • For reproduction of material from PCCP:
    [Original citation] - Published by the PCCP Owner Societies.
  • For reproduction of material from PPS:
    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
  • For reproduction of material from all other RSC journals:
    [Original citation] - Published by The Royal Society of Chemistry.

Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.


Social activity

Search articles by author

Spotlight

Advertisements