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Issue 63, 2014
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High throughput extraction of plasma using a secondary flow-aided inertial microfluidic device

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Abstract

In this paper, we report the development of a simple inertial microfluidic device with a serpentine channel for efficiently separating blood cells from plasma. The working mechanism of this device relies on the two-sided secondary flow aided inertial focusing of particles in a serpentine channel. Specifically, blood cells were focused along two sides of the channel, while the blood plasma was collected at the cell-free region within the channel centre. The device was tested with diluted (1/20) whole blood. A relatively high flow rate of 350 μl min−1 with a purity of ∼99.75% was achieved in a single process. A further improvement to 99.95% purity was obtained after a second process. Parallelization with eight parallel serpentine channels achieved a high flow rate of 2.8 ml min−1 and a massive throughput of 7 × 108 cells per min. Our device could be easily integrated with other sample preparation processes or detection units to form a sample-to-answer lab-on-a-chip system.

Graphical abstract: High throughput extraction of plasma using a secondary flow-aided inertial microfluidic device

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Publication details

The article was received on 02 Jul 2014, accepted on 17 Jul 2014 and first published on 18 Jul 2014


Article type: Paper
DOI: 10.1039/C4RA06513A
RSC Adv., 2014,4, 33149-33159

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    High throughput extraction of plasma using a secondary flow-aided inertial microfluidic device

    J. Zhang, S. Yan, W. Li, G. Alici and N. Nguyen, RSC Adv., 2014, 4, 33149
    DOI: 10.1039/C4RA06513A

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