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Issue 6, 2014
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The photophysics of LOV-based fluorescent proteins – new tools for cell biology

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Abstract

LOV-based fluorescent proteins (FPs) are an alternative class of fluorescent reporters with unique properties which complement the well-established proteins of the GFP family. One of the most important features of LOV-based FPs is the independence of molecular oxygen for the development of their specific fluorescence. Furthermore, they are characterized by small size and rapid signal development. Over the last few years, a number of different bacterial and plant LOV-based fluorescent proteins such as FbFP, iLOV and miniSOG have been developed and optimized. In this report, we comparatively have characterized the photophysical properties of nine different LOV-based fluorescent proteins including the excitation and emission maxima, the extinction coefficient, the fluorescence quantum yield, the average fluorescence lifetime and the photostability. The unified characterization of the LOV-based FPs provides a useful guide to apply them as in vivo tools for quantitative analyses and biological imaging.

Graphical abstract: The photophysics of LOV-based fluorescent proteins – new tools for cell biology

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Supplementary files

Article information


Submitted
02 Dec 2013
Accepted
19 Jan 2014
First published
23 Jan 2014

Photochem. Photobiol. Sci., 2014,13, 875-883
Article type
Paper
Author version available

The photophysics of LOV-based fluorescent proteins – new tools for cell biology

M. Wingen, J. Potzkei, S. Endres, G. Casini, C. Rupprecht, C. Fahlke, U. Krauss, K. Jaeger, T. Drepper and T. Gensch, Photochem. Photobiol. Sci., 2014, 13, 875
DOI: 10.1039/C3PP50414J

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