Jump to main content
Jump to site search

Issue 44, 2014
Previous Article Next Article

Templated DNA ligation with thiol chemistry

Author affiliations


We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner.

Graphical abstract: Templated DNA ligation with thiol chemistry

Back to tab navigation

Supplementary files

Article information

23 Jul 2014
24 Sep 2014
First published
06 Oct 2014

Org. Biomol. Chem., 2014,12, 8823-8827
Article type

Templated DNA ligation with thiol chemistry

D. Li, X. Wang, F. Shi, R. Sha, N. C. Seeman and J. W. Canary, Org. Biomol. Chem., 2014, 12, 8823
DOI: 10.1039/C4OB01552E

Social activity

Search articles by author