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Issue 44, 2014
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Templated DNA ligation with thiol chemistry

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Abstract

We describe two DNA-templated ligation strategies: native chemical ligation (NCL), and thiol-disulfide exchange. Both systems result in successful ligation in the presence of a DNA template. The stability of the product from the NCL reaction relies on exogenous thiol, while the thiol-disulfide reaction proceeds in a catalyst-free manner.

Graphical abstract: Templated DNA ligation with thiol chemistry

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Article information


Submitted
23 Jul 2014
Accepted
24 Sep 2014
First published
06 Oct 2014

Org. Biomol. Chem., 2014,12, 8823-8827
Article type
Communication

Templated DNA ligation with thiol chemistry

D. Li, X. Wang, F. Shi, R. Sha, N. C. Seeman and J. W. Canary, Org. Biomol. Chem., 2014, 12, 8823
DOI: 10.1039/C4OB01552E

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