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Issue 20, 2014
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Quantitative measurement of redox potential in hypoxic cells using SERS nanosensors

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Abstract

Hypoxia is considered to be a reductive disorder of cells that is caused either by a lack of oxygen or by the dysregulation of metabolic pathways and is thought to play a role in the pathology of diseases including stroke and cancer. One aspect of hypoxia that remains poorly investigated is the dysregulation of cellular redox potential and its role in controlling biological pathway activation. Since there is currently no way of quantitatively measuring the intracellular redox potential of hypoxic cells, this provided us with the motivation to develop optical nanosensors whose Surface-Enhanced Raman (SER) spectrum provides a quantitative measure of redox potential in hypoxic cells. Our nanosensors are made from organic reporter molecules that show oxidation-state-dependent changes in the Raman spectrum and are chemically adsorbed onto gold nanoshells. These nanosensors can be taken up by cells, and by collecting the SER spectrum we can calculate the localised intracellular redox potential from single hypoxic cells in a non-invasive, reversible way.

Graphical abstract: Quantitative measurement of redox potential in hypoxic cells using SERS nanosensors

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Supplementary files

Article information


Submitted
07 Mar 2014
Accepted
30 Jul 2014
First published
08 Sep 2014

This article is Open Access

Nanoscale, 2014,6, 12104-12110
Article type
Paper
Author version available

Quantitative measurement of redox potential in hypoxic cells using SERS nanosensors

J. Jiang, C. Auchinvole, K. Fisher and C. J. Campbell, Nanoscale, 2014, 6, 12104
DOI: 10.1039/C4NR01263A

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    [Original citation] - Published by The Royal Society of Chemistry.

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