Application of a new interface for rapid optimisation of bio-catalysed processes: proteolytic digestion and an enzyme-catalysed transesterification as examples
The results of an evaluation of the iChemExplorer for the study of bio-catalysed processes are reported. The iChemExplorer comprises of a specially-designed sample tray and a control unit, the former of which replaces a traditional tray in an HPLC autosampler assembly. It can be heated and reaction mixtures can be agitated. The system has been used to study the trypsin digestion of insulin chain B, cytochrome c and bovine serum albumin as well as the lipase-catalysed transesterification reaction between ethyl benzoate and 1-butanol.