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Issue 2, 2014
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Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches

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Abstract

The pacific oyster (Crassostrea gigas), a good model for studying biomineralization and coastal environmental change, was used to study the expression of cuproproteins after waterborne Cu exposure (3 μg Cu L−1) similarly to the Arcachon Bay aquatic environment. Cuproproteins in oyster cytosols were analyzed by gel electrophoresis (non-denaturing PAGE and SDS 2-DE), laser ablation inductively coupled plasma mass spectrometry (LA-ICP MS) and electrospray ionization mass spectrometry (ESI-MS). The high molecular weight cuproproteins (identified as extracellular superoxide dismutase [Cu–Zn] (EC Cu/Zn-SOD) and L-ascorbate oxidase) in digestive glands of oysters were differentially expressed after Cu exposure. About 1.7 and 1.2-fold increase of the Cu level in EC Cu/Zn-SOD and L-ascorbate oxidase were, respectively, determined, although EC Cu/Zn-SOD was 1.6–1.9-fold down-regulated in the digestive gland of oysters after Cu exposure. At last, the role of EC Cu/Zn-SOD in protection against Cu exposure was discussed to propose possible mechanisms to better understand Cu homeostasis and/or detoxification mechanisms.

Graphical abstract: Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches

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Publication details

The article was received on 19 Dec 2013, accepted on 06 Jan 2014 and first published on 07 Jan 2014


Article type: Paper
DOI: 10.1039/C3MT00375B
Metallomics, 2014,6, 338-346

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    Investigating the response of cuproproteins from oysters (Crassostrea gigas) after waterborne copper exposure by metallomic and proteomic approaches

    M. Xu, H. Bijoux, P. Gonzalez and S. Mounicou, Metallomics, 2014, 6, 338
    DOI: 10.1039/C3MT00375B

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