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Issue 1, 2014
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Intracellular co-delivery of zinc ions and plasmid DNA for enhancing gene transfection activity

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Abstract

Zinc ions, methylated poly(1-vinylimidazole) (PVIm-Me) and plasmid DNA (pDNA) have formed ternary complexes for gene delivery. The resulting Zn–PVIm-Me–pDNA complexes have delivered both Zn2+ ions and pDNA inside cells, leading to the nuclear translocation of the pDNA. By use of the pDNA containing a nuclear protein, NF-κB, binding sequence, the intracellular co-delivery of Zn2+ ions and pDNA has enhanced gene expression. These results suggest that the intracellular Zn2+ ions delivered by Zn–PVIm-Me–pDNA complexes activated the NF-κB, enhancing the nuclear translocation of the pDNA. In conclusion, it has been demonstrated that the Zn–PVIm-Me–pDNA complex is capable of enhancing the gene transfection activity by a synergic effect of the PVIm-Me and the co-delivered intracellular Zn2+ ions.

Graphical abstract: Intracellular co-delivery of zinc ions and plasmid DNA for enhancing gene transfection activity

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Publication details

The article was received on 20 Aug 2013, accepted on 19 Sep 2013 and first published on 19 Sep 2013


Article type: Paper
DOI: 10.1039/C3MT00226H
Metallomics, 2014,6, 82-87

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    Intracellular co-delivery of zinc ions and plasmid DNA for enhancing gene transfection activity

    S. Asayama, K. Matsuda, Y. Negishi and H. Kawakami, Metallomics, 2014, 6, 82
    DOI: 10.1039/C3MT00226H

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