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Issue 19, 2014
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Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter

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Abstract

We describe a versatile microfluidic fluorescence-activated cell sorter that uses acoustic actuation to sort cells or drops at ultra-high rates. Our acoustic sorter combines the advantages of traditional fluorescence-activated cell (FACS) and droplet sorting (FADS) and is applicable for a multitude of objects. We sort aqueous droplets, at rates as high as several kHz, into two or even more outlet channels. We can also sort cells directly from the medium without prior encapsulation into drops; we demonstrate this by sorting fluorescently labeled mouse melanoma cells in a single phase fluid. Our acoustic microfluidic FACS is compatible with standard cell sorting cytometers, yet, at the same time, enables a rich variety of more sophisticated applications.

Graphical abstract: Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter

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Supplementary files

Article information


Submitted
20 May 2014
Accepted
30 Jun 2014
First published
30 Jun 2014

Lab Chip, 2014,14, 3710-3718
Article type
Paper
Author version available

Sorting drops and cells with acoustics: acoustic microfluidic fluorescence-activated cell sorter

L. Schmid, D. A. Weitz and T. Franke, Lab Chip, 2014, 14, 3710
DOI: 10.1039/C4LC00588K

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