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Issue 15, 2014
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Controllable electrofusion of lipid vesicles: initiation and analysis of reactions within biomimetic containers

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Abstract

We present a microfluidic device that is able to trap multiple giant unilamellar vesicles (GUVs) and initiate electrofusion via integrated microelectrodes. PDMS posts were designed to trap and isolate two or more vesicles. Electrodes patterned onto the glass surface of the microchannels are able to apply a short, high voltage pulse across the traps for controllable electrofusion of the GUVs. The entire array of traps and electrodes are designed such that an average of 60 individual fusion experiments can be performed on-chip. An assay based on Förster resonance energy transfer (FRET) is performed to show successful lipid mixing. Not only can the device be used to record the dynamics of lipid membrane fusion, but it can be used for reaction monitoring by fusing GUVs containing reactants. We demonstrate this by fusing vesicles encapsulating femtolitre volumes of cobalt chloride or EDTA and monitoring the amount of the complexation product over time.

Graphical abstract: Controllable electrofusion of lipid vesicles: initiation and analysis of reactions within biomimetic containers

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Publication details

The article was received on 17 Apr 2014, accepted on 28 May 2014 and first published on 29 May 2014


Article type: Paper
DOI: 10.1039/C4LC00460D
Lab Chip, 2014,14, 2852-2859

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    Controllable electrofusion of lipid vesicles: initiation and analysis of reactions within biomimetic containers

    T. Robinson, P. E. Verboket, K. Eyer and P. S. Dittrich, Lab Chip, 2014, 14, 2852
    DOI: 10.1039/C4LC00460D

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