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Issue 37, 2014
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Novel in situ methodology to observe the interactions of chemotherapeutical Pt drugs with DNA under physiological conditions

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Abstract

The binding of the antitumor drug cisplatin with DNA was determined by means of in situ resonant inelastic X-ray scattering (RIXS) spectroscopy. Because of the penetrating properties of hard X-rays, we could determine, under physiological conditions, the identity and number of platinum complexes present. In situ RIXS revealed that under physiological conditions, water molecules replace chloride ligands owing to drug hydration. The subsequent interaction with DNA, led to the bonding of the aqua complexes into the DNA structure with simultaneous loss of the coordinating water and chloride ion. The data analysis reveals that Pt is coordinated by two adjacent guanines giving cis-[Pt(NH3)2{d(GpG)-N7(1),-N7(2)}] upon losing its coordinating water or chloride ligands.

Graphical abstract: Novel in situ methodology to observe the interactions of chemotherapeutical Pt drugs with DNA under physiological conditions

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Article information


Submitted
22 Mar 2014
Accepted
30 Apr 2014
First published
02 May 2014

Dalton Trans., 2014,43, 13839-13844
Article type
Paper
Author version available

Novel in situ methodology to observe the interactions of chemotherapeutical Pt drugs with DNA under physiological conditions

E. Lipiec, J. Czapla, J. Szlachetko, Y. Kayser, W. Kwiatek, B. Wood, G. B. Deacon and J. Sá, Dalton Trans., 2014, 43, 13839
DOI: 10.1039/C4DT00861H

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