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Issue 22, 2014
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Complexation of DNA with ruthenium organometallic compounds: the high complexation ratio limit

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Abstract

Interactions between DNA and ruthenium organometallic compounds are studied by using visible light absorption and circular dichroism measurements. A titration technique allowing for the absolute determination of the advancement degree of the complexation, without any assumption about the number of complexation modes is developed. When DNA is in excess, complexation involves intercalation of one of the organometallic compound ligands between DNA base pairs. But, in the high complexation ratio limit, where organometallic compounds are in excess relative to the DNA base pairs, a new mode of interaction is observed, in which the organometallic compound interacts weakly with DNA. The weak interaction mode, moreover, develops when all the DNA intercalation sites are occupied. A regime is reached in which one DNA base pair is linked to more than one organometallic compound.

Graphical abstract: Complexation of DNA with ruthenium organometallic compounds: the high complexation ratio limit

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Article information


Submitted
18 Feb 2014
Accepted
27 Mar 2014
First published
28 Mar 2014

Phys. Chem. Chem. Phys., 2014,16, 10491-10502
Article type
Paper
Author version available

Complexation of DNA with ruthenium organometallic compounds: the high complexation ratio limit

S. Despax, F. Jia, M. Pfeffer and P. Hébraud, Phys. Chem. Chem. Phys., 2014, 16, 10491
DOI: 10.1039/C4CP00726C

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