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Issue 81, 2014
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Chemical biology of genomic DNA: minimizing PCR bias

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Abstract

The exquisite selectivity of chemical reactions enables the study of rare DNA bases. However, chemical modification of the genome can affect downstream analysis. We report a PCR bias caused by such modification, and exemplify a solution with the synthesis and characterization of a cleavable aldehyde-reactive biotinylation probe.

Graphical abstract: Chemical biology of genomic DNA: minimizing PCR bias

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Article information


Submitted
03 Jul 2014
Accepted
15 Aug 2014
First published
18 Aug 2014

This article is Open Access

Chem. Commun., 2014,50, 12047-12049
Article type
Communication
Author version available

Chemical biology of genomic DNA: minimizing PCR bias

G. R. McInroy, E. Raiber and S. Balasubramanian, Chem. Commun., 2014, 50, 12047
DOI: 10.1039/C4CC05107F

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