Jump to main content
Jump to site search

Issue 78, 2014
Previous Article Next Article

Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding

Author affiliations

Abstract

Fluorescent protein biosensors are typically implemented via genetic encoding which makes the examination of scarce cell samples impractical. By directly delivering the protein form of the biosensor into cells using electroporation, we detected intracellular molecular activity with the sample size down to ∼100 cells with high spatiotemporal resolution.

Graphical abstract: Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Jun 2014, accepted on 07 Aug 2014 and first published on 08 Aug 2014


Article type: Communication
DOI: 10.1039/C4CC04730C
Chem. Commun., 2014,50, 11536-11539
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Electroporation-delivered fluorescent protein biosensors for probing molecular activities in cells without genetic encoding

    C. Sun, M. Ouyang, Z. Cao, S. Ma, H. Alqublan, N. Sriranganathan, Y. Wang and C. Lu, Chem. Commun., 2014, 50, 11536
    DOI: 10.1039/C4CC04730C

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements