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Issue 74, 2014
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Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels–Alder reaction

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Abstract

Many applications in biotechnology and molecular biology rely on modified nucleotides. Here, we present an approach for the postsynthetic labelling of enzymatically synthesized vinyl-modified DNA by Diels–Alder reaction with inverse electron demand using a tetrazine. Labelling proceeds very efficiently and supersedes several known approaches.

Graphical abstract: Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels–Alder reaction

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Publication details

The article was received on 06 Jun 2014, accepted on 11 Jul 2014 and first published on 22 Jul 2014


Article type: Communication
DOI: 10.1039/C4CC04332D
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Chem. Commun., 2014,50, 10827-10829
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    Efficient labelling of enzymatically synthesized vinyl-modified DNA by an inverse-electron-demand Diels–Alder reaction

    H. Bußkamp, E. Batroff, A. Niederwieser, O. S. Abdel-Rahman, R. F. Winter, V. Wittmann and A. Marx, Chem. Commun., 2014, 50, 10827
    DOI: 10.1039/C4CC04332D

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