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Issue 59, 2014
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Native chemical ubiquitination using a genetically incorporated azidonorleucine

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Abstract

A robust chemical ubiquitination method was developed. The method employed a genetically incorporated azidonorleucine as an orthogonal lysine precursor for the installation of a Gly residue bearing an Nα-auxiliary which mediated the ligation between ubiquitin(1–75)-thioester and the target protein. To demonstrate our methodology, a model protein, K48-linked diubiquitin, was synthesized with an overall yield of 35%.

Graphical abstract: Native chemical ubiquitination using a genetically incorporated azidonorleucine

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Supplementary files

Article information


Submitted
15 May 2014
Accepted
04 Jun 2014
First published
04 Jun 2014

Chem. Commun., 2014,50, 7971-7974
Article type
Communication
Author version available

Native chemical ubiquitination using a genetically incorporated azidonorleucine

R. Yang, X. Bi, F. Li, Y. Cao and C. Liu, Chem. Commun., 2014, 50, 7971
DOI: 10.1039/C4CC03721A

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