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Issue 10, 2014
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AFM analysis of changes in nucleosome wrapping induced by DNA epigenetic modification

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Abstract

The wrapping and unwrapping of the nucleosome, which is a fundamental packing unit of chromatin, are tied to the regulation of gene expression. The accessibility of DNA within nucleosomes is controlled not only by chromatin-remodeling molecules, but also by chemical modifications of histones and DNA. Understanding the structural changes of a nucleosome during epigenetic modifications is a key to unravel the mechanisms of gene regulation. Here, we reconstituted nucleosomes using methylcytosine- or hydroxymethylcytosine-substituted DNA, and analyzed their morphological features by atomic force microscopy (AFM). Our results indicate that cytosine methylation induces overwrapping of the DNA around the histone octamer, whereas cytosine hydroxymethylation has a lesser effect on the overwrapping of the DNA. These results suggest that two types of DNA modification yield different wrapping states of nucleosomes, which may contribute to the compaction and relaxation of the chromatin structure.

Graphical abstract: AFM analysis of changes in nucleosome wrapping induced by DNA epigenetic modification

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Publication details

The article was received on 09 Apr 2014, accepted on 13 Jun 2014 and first published on 30 Jun 2014


Article type: Paper
DOI: 10.1039/C4BM00113C
Biomater. Sci., 2014,2, 1399-1403

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    AFM analysis of changes in nucleosome wrapping induced by DNA epigenetic modification

    S. Kizaki, Y. Suzuki, T. Takenaka, M. Endo and H. Sugiyama, Biomater. Sci., 2014, 2, 1399
    DOI: 10.1039/C4BM00113C

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