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Issue 6, 2014
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Stabilization of a hyaluronate-associated gene delivery system using calcium ions

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Abstract

A “DPH” ternary complex consisting of plasmid DNA (pDNA), intracellularly degradable polyethyleneimine, and hyaluronic acid (HA) is a promising non-viral gene carrier with low toxicity and good gene transfection efficiency. HA plays a key role in providing an optimal balance between DNA protection and release, but it causes aggregation due to the entanglement of HA chains of neighbouring DPH particles. Here we report that the addition of an optimal level of Ca2+ successfully prevents particle aggregation and maintains a relatively small size. The Ca-stabilized DPH is comparable to DPH in cytotoxicity and gene transfection efficiency. MW monitoring and conductometric titration suggest that such size stabilization effect is partly mediated by the complexation between HA and Ca2+, which enables intra- and intermolecular interactions of HAs.

Graphical abstract: Stabilization of a hyaluronate-associated gene delivery system using calcium ions

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Supplementary files

Article information


Submitted
12 Jan 2014
Accepted
06 Feb 2014
First published
19 Feb 2014

Biomater. Sci., 2014,2, 936-942
Article type
Paper
Author version available

Stabilization of a hyaluronate-associated gene delivery system using calcium ions

M. Feng, B. M. Ibrahim, E. M. Wilson, K. Doh, B. K. Bergman, C. Park and Y. Yeo, Biomater. Sci., 2014, 2, 936
DOI: 10.1039/C4BM00012A

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