Issue 16, 2014
From the journal:

Analytical Methods

Electrochemical evaluation of sarcomeric α-actinin in embryoid bodies after gene silencing using an LSI-based amperometric sensor array†

Mustafa Şen,a   Kosuke Ino,*a   Kumi Y. Inoue,a   Atsushi Suda,b   Ryota Kunikata,b   Masahki Matsudaira,c   Hitoshi Shikua  and  Tomokazu Matsue*ad  

* Corresponding authors

a Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan
E-mail: ino.kosuke@bioinfo.che.tohoku.ac.jp
Fax: +81-22-795-7281
Tel: +81-22-795-7281

b Japan Aviation Electronics Industry, Ltd., Tokyo 196-8555, Japan

c Micro System Integration Center, Tohoku University, Sendai 980-0845, Japan

d WPI-Advanced Institute for Materials Research, Tohoku University, Sendai 980-8577, Japan
E-mail: matsue@bioinfo.che.tohoku.ac.jp

Abstract

In the present study, gene analysis using siRNAs for cell differentiation of embryonic stem (ES) cells was performed using an LSI-based amperometric sensor array (Bio-LSI). CITED2 and WNT11 were silenced using siRNA, and then the effect of these genes on the differentiation of ES cells into cardiomyocytes was evaluated. For the evaluation, endogenous ALP activity and sarcomeric α-actinin were electrochemically detected using the Bio-LSI system. These results show the crucial role of these genes in determining the fate of differentiation of ES cells. These results also show that the Bio-LSI is a great tool for cell analysis.

Graphical abstract: Electrochemical evaluation of sarcomeric α-actinin in embryoid bodies after gene silencing using an LSI-based amperometric sensor array

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Supplementary files

Article information

DOI
https://doi.org/10.1039/C4AY00791C
Article type
Paper
Submitted
01 Apr 2014
Accepted
10 Apr 2014
First published
11 Apr 2014

Anal. Methods, 2014,6, 6337-6342

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Electrochemical evaluation of sarcomeric α-actinin in embryoid bodies after gene silencing using an LSI-based amperometric sensor array

M. Şen, K. Ino, K. Y. Inoue, A. Suda, R. Kunikata, M. Matsudaira, H. Shiku and T. Matsue, Anal. Methods, 2014, 6, 6337 DOI: 10.1039/C4AY00791C

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