Jump to main content
Jump to site search

Issue 9, 2014
Previous Article Next Article

A sensitive electrochemical biosensor for microRNA detection based on streptavidin–gold nanoparticles and enzymatic amplification

Author affiliations

Abstract

An electrochemical method for microRNA (miRNA) detection has been proposed with a dual signal amplification strategy. The method relies on polymerase extension and a two-step signal amplification using streptavidin–gold nanoparticle biocomplexes and alkaline phosphatase. The target miRNA can hybridize with the capture DNA template, which can act as a primer and be extended along the template in the presence of DNA polymerase and dNTPs. A biotin group was introduced into the duplex by the incorporation of biotin-11-dUTP. Thus, biotinylated alkaline phosphatase would bind to the duplex using streptavidin–gold nanoparticles as linkers, which resulted in an amplified electrochemical signal. The electrochemical signal exhibited a linear correlation to the logarithm of miRNA concentration ranging from 100 fM to 1 nM, with a detection limit of 99.2 fM. The specificity of the method allowed single-nucleotide differences between miRNA family members to be discriminated. The established biosensor displayed an excellent analytical performance towards miRNA detection and might present a convenient tool for biomedical research and clinical diagnostic applications.

Graphical abstract: A sensitive electrochemical biosensor for microRNA detection based on streptavidin–gold nanoparticles and enzymatic amplification

Back to tab navigation

Publication details

The article was received on 06 Jan 2014, accepted on 28 Jan 2014 and first published on 28 Jan 2014


Article type: Paper
DOI: 10.1039/C4AY00033A
Author version
available:
Download author version (PDF)
Anal. Methods, 2014,6, 2889-2893

  •   Request permissions

    A sensitive electrochemical biosensor for microRNA detection based on streptavidin–gold nanoparticles and enzymatic amplification

    Y. Peng, J. Jiang and R. Yu, Anal. Methods, 2014, 6, 2889
    DOI: 10.1039/C4AY00033A

Search articles by author

Spotlight

Advertisements