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Issue 15, 2014
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Determination of parabens in human urine by liquid chromatography coupled with electrospray ionization tandem mass spectrometry

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Abstract

A simple and sensitive method was developed for the simultaneous determination of methyl, ethyl, n-propyl, n-butyl, and benzyl parabens in human urine by liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). Enzymatic hydrolysis conditions were optimized to deconjugate the urinary parabens, glucuronide and sulfate conjugates. Solid phase extraction (SPE) was then used for sample clean-up. LC-ESI-MS/MS conditions for sample analysis were also optimized to achieve the maximal sensitivity and accuracy. Parabens were finally separated on a C8 reversed phase column. Correlation coefficients (R2) and recoveries ranged from 0.998 to 0.999 and 80.6% to 95.6%, respectively, and intra-day and inter-day precisions (relative standard deviation, RSD) were within 1.2–4.5% and 2.2–7.1%, respectively. Limits of detection (LODs) for methyl, ethyl, n-propyl, n-butyl, and benzyl parabens were 3, 3, 3, 3, and 1 pg, respectively. The optimized method was successfully used to determine parabens in urine samples from school students in Southern China.

Graphical abstract: Determination of parabens in human urine by liquid chromatography coupled with electrospray ionization tandem mass spectrometry

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Publication details

The article was received on 19 Nov 2013, accepted on 15 May 2014 and first published on 30 May 2014


Article type: Paper
DOI: 10.1039/C3AY42063A
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Anal. Methods, 2014,6, 5566-5572

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    Determination of parabens in human urine by liquid chromatography coupled with electrospray ionization tandem mass spectrometry

    S. Lu, S. Gong, S. Ma, X. Zeng, Z. Yu, G. Sheng and J. Fu, Anal. Methods, 2014, 6, 5566
    DOI: 10.1039/C3AY42063A

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