Jump to main content
Jump to site search

Issue 1, 2015
Previous Article Next Article

In vitro HER2 protein-induced affinity dissociation of carbon nanotube-wrapped anti-HER2 aptamers for HER2 protein detection

Author affiliations

Abstract

A new in vitro assay was developed to detect human epidermal growth factor receptor 2 (HER2) protein, based on affinity dissociation of carbon nanotube (CNT)-wrapped anti-HER2 ssDNA aptamers. First, we selected an anti-HER2 ssDNA aptamer (H2) using an in vitro serial evolution of ligands by an exponential enrichment (SELEX) process. Then the fluorescently labelled H2 ssDNAs were tightly packed on CNTs that had previously been coupled with magnetic microbeads (MBs), forming MB–CNT–H2 hybrids. The loading capacity of these MB–CNTs heterostructures (2.8 × 108) was determined to be 0.025 to 3.125 μM of H2. HER2 protein-induced H2 dissociation occurred from MB–CNT–H2 hybrids, which was specifically induced by the target HER2 protein, with a dissociation constant (Kd) of 270 nM. The stoichiometric affinity dissociation ratio with respect to H2-to-HER2 protein was shown to be approximately 1 : 1. Our results demonstrated that the developed assay can be an effective approach in detecting native forms of disease biomarkers in free solutions or in biological samples, for accurate diagnosis.

Graphical abstract: In vitro HER2 protein-induced affinity dissociation of carbon nanotube-wrapped anti-HER2 aptamers for HER2 protein detection

Back to tab navigation

Supplementary files

Publication details

The article was received on 10 Sep 2014, accepted on 16 Oct 2014 and first published on 16 Oct 2014


Article type: Paper
DOI: 10.1039/C4AN01665C
Author version
available:
Download author version (PDF)
Citation: Analyst, 2015,140, 243-249

  •   Request permissions

    In vitro HER2 protein-induced affinity dissociation of carbon nanotube-wrapped anti-HER2 aptamers for HER2 protein detection

    J. H. Niazi, S. K. Verma, S. Niazi and A. Qureshi, Analyst, 2015, 140, 243
    DOI: 10.1039/C4AN01665C

Search articles by author

Spotlight

Advertisements