Issue 23, 2014

A novel molecularly imprinted chitosan–acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin

Abstract

A novel highly sensitive and selective molecularly imprinted polymer (MIP) cryogel biosensor for determination of microalbumin in urine samples was fabricated. The MIP gel was prepared based on the graft copolymerization of acrylamide with N,N′-methylenebisacrylamide on chitosan using human serum albumin (HSA) as the template. The sub-zero polymerization allowed the solvent to form ice crystals and left a macroporous cryogel structure when it was thawed. After removing the template, the specific imprinted surface on cryogel pore walls was used to detect HSA via a redox mediator (ferrocene), entrapped in the cryogel, using differential pulse voltammetry (DPV). The electrochemical detection was improved by the presence of graphene that has been composited within the polymer. For determination of albumin, the fabricated MIP cryogel biosensor showed a high sensitivity with a wide linear range of 1.0 × 10−4 to 1.0 × 101 mg L−1 and a low limit of detection of 5.0 × 10−5 mg L−1 (S/N = 3). The sensor also provided a very good reusability, i.e., the sensitivity remained >90% after 9 cycles of binding–rewashing (18 analyses per cycle), while the sensitivity only decreased to 90% after 6 weeks of storage at room temperature. The biosensor also showed a good selectivity, both against bovine serum albumin (BSA) and some common possible interfering compounds normally present in urine (ascorbic acid, uric acid, urea, sodium, chloride, potassium and creatinine). The excellent performance of the biosensor was confirmed by analyzing microalbumin in urine samples, and results were in good agreement with those obtained by the standard immunoturbidimetric method (P > 0.05).

Graphical abstract: A novel molecularly imprinted chitosan–acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin

Article information

Article type
Paper
Submitted
02 Jun 2014
Accepted
20 Sep 2014
First published
22 Sep 2014

Analyst, 2014,139, 6160-6167

A novel molecularly imprinted chitosan–acrylamide, graphene, ferrocene composite cryogel biosensor used to detect microalbumin

A. Fatoni, A. Numnuam, P. Kanatharana, W. Limbut and P. Thavarungkul, Analyst, 2014, 139, 6160 DOI: 10.1039/C4AN01000K

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements