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Issue 18, 2014
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Electrochemical enzymatic biosensor with long-term stability using hybrid mesoporous membrane

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Abstract

An acetylcholinesterase-immobilized sensor unit was successfully prepared by encapsulating the enzyme within hybrid mesoporous silica membranes (F127-MST). Through a novel combination with tetracyanoquinodimethane, both acetylcholine and organophosphorus pesticides were successfully detected with high sensitivity. Furthermore, we manufactured the working prototype of an enzyme sensor with this sensor unit for detecting dichlorvos, aldicarb and parathion. At present, the detection limit in this working prototype either equaled or surpassed that of others. Also, we have the advantage of increased stability of the enzyme against the outer environment by encapsulation of the enzymes into a silica nanospace. Consequently, acetylcholinesterase immobilized in F127-MST is a practical sensor with high sensitivity, reusability, and storage stability.

Graphical abstract: Electrochemical enzymatic biosensor with long-term stability using hybrid mesoporous membrane

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Article information


Submitted
29 May 2014
Accepted
30 Jun 2014
First published
01 Jul 2014

Analyst, 2014,139, 4654-4660
Article type
Paper
Author version available

Electrochemical enzymatic biosensor with long-term stability using hybrid mesoporous membrane

T. Itoh, T. Shimomura, A. Hayashi, A. Yamaguchi, N. Teramae, M. Ono, T. Tsunoda, F. Mizukami, G. D. Stucky and T. Hanaoka, Analyst, 2014, 139, 4654
DOI: 10.1039/C4AN00975D

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