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Issue 12, 2014
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Simple sample processing enhances malaria rapid diagnostic test performance

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Abstract

Lateral flow immunochromatographic rapid diagnostic tests (RDTs) are the primary form of medical diagnostic used for malaria in underdeveloped nations. Unfortunately, many of these tests do not detect asymptomatic malaria carriers. In order for eradication of the disease to be achieved, this problem must be solved. In this study, we demonstrate enhancement in the performance of six RDT brands when a simple sample-processing step is added to the front of the diagnostic process. Greater than a 4-fold RDT signal enhancement was observed as a result of the sample processing step. This lowered the limit of detection for RDT brands to submicroscopic parasitemias. For the best performing RDTs the limits of detection were found to be as low as 3 parasites per μL. Finally, through individual donor samples, the correlations between donor source, WHO panel detection scores and RDT signal intensities were explored.

Graphical abstract: Simple sample processing enhances malaria rapid diagnostic test performance

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Supplementary files

Article information


Submitted
17 Feb 2014
Accepted
17 Apr 2014
First published
17 Apr 2014

This article is Open Access

Analyst, 2014,139, 3026-3031
Article type
Paper
Author version available

Simple sample processing enhances malaria rapid diagnostic test performance

K. M. Davis, L. E. Gibson, F. R. Haselton and D. W. Wright, Analyst, 2014, 139, 3026
DOI: 10.1039/C4AN00338A

This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material.

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    [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
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    [Original citation] - Published by The Royal Society of Chemistry.

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