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Issue 10, 2014
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Enhancing permanganate chemiluminescence detection for the determination of glutathione and glutathione disulfide in biological matrices

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Abstract

Acidic potassium permanganate chemiluminescence enables direct post-column detection of glutathione, but its application to assess the redox state of a wider range of biological fluids and tissues is limited by its sensitivity. Herein we show that the simple on-line addition of an aqueous formaldehyde solution not only enhances the sensitivity of the procedure by two orders of magnitude, but also provides a remarkable improvement in the selectivity of the reagent towards thiols such as glutathione (compared to phenols and amino acids that do not possess a thiol group). This enhanced mode of detection was applied to the determination of glutathione and its corresponding disulfide species in homogenised striatum samples taken from both wild type mice and the R6/1 transgenic mouse model of Huntington's disease, at both 8 and 12 weeks of age. No significant difference was observed between the GSH/GSSG ratios of wild type mice and R6/1 mice at either age group, suggesting that the early disease progression had not significantly altered the intracellular redox environment.

Graphical abstract: Enhancing permanganate chemiluminescence detection for the determination of glutathione and glutathione disulfide in biological matrices

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Supplementary files

Article information


Submitted
05 Feb 2014
Accepted
24 Mar 2014
First published
25 Mar 2014

This article is Open Access

Analyst, 2014,139, 2416-2422
Article type
Paper
Author version available

Enhancing permanganate chemiluminescence detection for the determination of glutathione and glutathione disulfide in biological matrices

Z. M. Smith, J. M. Terry, N. W. Barnett, L. J. Gray, D. J. Wright and P. S. Francis, Analyst, 2014, 139, 2416
DOI: 10.1039/C4AN00255E

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