Issue 10, 2014

Recent advances in sample preparation techniques to overcome difficulties encountered during quantitative analysis of small molecules from biofluids using LC-MS/MS

Abstract

Liquid chromatography-mass spectrometry analysis of small molecules from biofluids requires sensitive and robust assays. Because of the very complex nature of many biological samples, efficient sample preparation protocols to remove unwanted components and to selectively extract the compounds of interest are an essential part of almost every bioanalytical workflow. This review describes the most common problems encountered during sample preparation, ways to optimize established sample preparation techniques and important recent developments to reduce or eliminate major interferents from biofluids.

Graphical abstract: Recent advances in sample preparation techniques to overcome difficulties encountered during quantitative analysis of small molecules from biofluids using LC-MS/MS

Article information

Article type
Minireview
Submitted
14 Jan 2014
Accepted
12 Feb 2014
First published
13 Feb 2014
This article is Open Access
Creative Commons BY license

Analyst, 2014,139, 2265-2276

Recent advances in sample preparation techniques to overcome difficulties encountered during quantitative analysis of small molecules from biofluids using LC-MS/MS

C. Bylda, R. Thiele, U. Kobold and D. A. Volmer, Analyst, 2014, 139, 2265 DOI: 10.1039/C4AN00094C

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