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Issue 10, 2014
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Electrochemiluminescence resonance energy transfer between graphene quantum dots and gold nanoparticles for DNA damage detection

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Abstract

Bright blue luminescent graphene quantum dots (GQDs) with major graphitic structured nanocrystals and a photoluminescence (PL) quantum yield of 15.5% were synthesized and used to monitor DNA damage. The GQDs were prepared by ultraviolet irradiation without using a chemical agent. The as-prepared GQDs showed excitation-dependent PL and stable electrochemiluminescence (ECL) behaviors. Gold nanoparticles (AuNPs) were linked with a probe of single-stranded DNA (cp53 ssDNA) to form AuNPs-ssDNA. The ECL signal of the GQDs could be quenched by non-covalent binding of the AuNPs-ssDNA to the GQDs, due to the occurrence of an electrochemiluminescence resonance energy transfer between the GQDs and the AuNPs. When AuNPs-ssDNA was then hybridized with target p53 DNA to form AuNPs-dsDNA, the non-covalent interaction between the GQDs and the ds-DNA weakened and the ECL of the GQDs recovered. This engendered an ECL sensor for the detection of target p53 ssDNA, with a detection limit of 13 nM. The resultant ECL sensor could be used for DNA damage detection based on its different bonding ability to damaged target p53 ssDNA and cp53 ssDNA linked AuNPs. The presented method could be expanded to the development of other ECL biosensors, for the quantification of nucleic acids, single nucleotide polymorphisms or other aptamer-specific biomolecules.

Graphical abstract: Electrochemiluminescence resonance energy transfer between graphene quantum dots and gold nanoparticles for DNA damage detection

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Supplementary files

Article information


Submitted
06 Jan 2014
Accepted
07 Feb 2014
First published
07 Feb 2014

Analyst, 2014,139, 2404-2410
Article type
Paper
Author version available

Electrochemiluminescence resonance energy transfer between graphene quantum dots and gold nanoparticles for DNA damage detection

Q. Lu, W. Wei, Z. Zhou, Z. Zhou, Y. Zhang and S. Liu, Analyst, 2014, 139, 2404
DOI: 10.1039/C4AN00020J

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