Convenient detection of the thiol functional group using H/D isotope sensitive Raman spectroscopy†
Abstract
Raman spectra of several thiols (amino acids, peptides and organic) show that the C–S–H bending mode (βCSH) shifts from ∼850 cm−1 to ∼620 cm−1 on deuteration of the thiol proton by simply dissolving them in D2O and CD3OD where detection by 1H NMR is not possible. A nondestructive analytical tool for the detection of thiols in solid/neat and in solution is developed.