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Issue 6, 2014
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Protein binding for detection of small changes on a nanoparticle surface

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Abstract

Protein adsorption on nanoparticles is closely associated with the physicochemical properties of particles, in particular, their surface properties. We synthesized two batches of polyacrylic acid-coated nanoparticles under almost identical conditions except for the heating duration and found differences in the head-group structure of the polyacrylic acid. The structure change was confirmed by NMR and MS. The two batches of particles had varied binding affinities to a selected group of proteins. Computational work confirmed that the head group of the polymer on the surface of a nanoparticle could directly interact with a protein, and small structural changes in the head group were sufficient to result in a significant difference in the free energy of binding. Our results demonstrate that protein adsorption is so sensitive to the surface properties of particles that it can reveal even small variations in the structure of a nanoparticle surface ligand, and should be useful for quick assessment of nanoparticle properties.

Graphical abstract: Protein binding for detection of small changes on a nanoparticle surface

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Article information


Submitted
20 Nov 2013
Accepted
30 Dec 2013
First published
03 Jan 2014

Analyst, 2014,139, 1364-1371
Article type
Paper
Author version available

Protein binding for detection of small changes on a nanoparticle surface

S. Zeng, Y. M. Huang, C. A. Chang and W. Zhong, Analyst, 2014, 139, 1364
DOI: 10.1039/C3AN02155F

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