Jump to main content
Jump to site search

Issue 23, 2013
Previous Article Next Article

Microvascular endothelial cell spreading and proliferation on nanofibrous scaffolds by polymer blends with enhanced wettability

Author affiliations

Abstract

The objective of this study is elucidating the mechanisms by which the wettability of nanofibrous electrospun mats varies in polymer blends, and highlighting how this can play a pivotal role in enhancing the viability of cultured microvascular endothelial cells (EC). A functional microvascular network is essential for supplying bioengineered tissues with oxygen and nutrients while removing metabolic wastes. An in vitro pre-vascularization strategy consists of seeding EC on scaffolds, which in turn promotes cells infiltration, adhesion and functionality. We use electrospun poly-L-lactic acid (PLLA) and gelatin (Gel) as prototype materials for realizing nanofibrous scaffolds as bioartificial architectures to improve the proliferation and the functionality of human microvascular ECs (HMEC-1). HMEC-1 seeded on electrospun scaffolds adhere, remain viable, proliferate and positively express the endothelial cell marker CD31 particularly on blend PLLA/Gel fibers, which exhibit wettability enhanced with respect to both the constituent polymers, and are therefore especially promising constructs for promoting the formation of functional endothelial tissue. The wettability characteristics of the blend polymer fibrous scaffolds are modeled and discussed. These results can be valuable for the future design of pre-vascularized scaffolds with enhanced wettability properties for functional tissue engineered implants, with ECs able to form in perspective an effectively functioning vasculature upon implantation.

Graphical abstract: Microvascular endothelial cell spreading and proliferation on nanofibrous scaffolds by polymer blends with enhanced wettability

Back to tab navigation

Publication details

The article was received on 31 Jan 2013, accepted on 21 Mar 2013 and first published on 23 Apr 2013


Article type: Paper
DOI: 10.1039/C3SM50328C
Citation: Soft Matter, 2013,9, 5529-5539
  •   Request permissions

    Microvascular endothelial cell spreading and proliferation on nanofibrous scaffolds by polymer blends with enhanced wettability

    M. Moffa, A. Polini, A. G. Sciancalepore, L. Persano, E. Mele, L. G. Passione, G. Potente and D. Pisignano, Soft Matter, 2013, 9, 5529
    DOI: 10.1039/C3SM50328C

Search articles by author

Spotlight

Advertisements