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Issue 1, 2013
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Digital photography for the analysis of fluorescence responses

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Abstract

Different pyridine substituted cruciform fluorophores (XF) were prepared and their optical properties were studied. Upon protonation of the pyridine nitrogen, the XFs with a para or ortho substitution pattern show similar bathochromic shifts in the emission, whereas the meta XF features a smaller bathochromic shift in emission wavelength. The color changes of XFs in the presence of carboxylic acids were used to identify them by digital photography. Data extraction of the photographs is performed by a combination of a large RGB color space, a standard white balance, the transformation of RGB values into uv′ values and MANOVA statistics, improving the recognition of analytes by pyridine XFs. We discuss in this paper factors that determine the quality of the extraction of color information from digital photograph.

Graphical abstract: Digital photography for the analysis of fluorescence responses

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Supplementary files

Article information


Submitted
01 Sep 2012
Accepted
16 Oct 2012
First published
17 Oct 2012

Chem. Sci., 2013,4, 273-281
Article type
Edge Article

Digital photography for the analysis of fluorescence responses

T. Schwaebel, O. Trapp and U. H. F. Bunz, Chem. Sci., 2013, 4, 273
DOI: 10.1039/C2SC21412A

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