Jump to main content
Jump to site search

Issue 26, 2013
Previous Article Next Article

Fabrication of albumin-micropatterned surfaces by colloidal microcontact printing technique

Author affiliations

Abstract

We report a versatile method for selective patterning of solid surfaces with albumin by a combination of microcontact printing of polymer colloids and surface charge aided protein adsorption. The technique allows formation of biocomposite arrays including colloid particles with different albumin functionalities. Micropatterns of functionalised monodisperse colloid particles were formed on the solid surfaces by using a microcontact printing method. We used polyelectrolyte multilayers (PEMs) as a coating system for poly (methylmethacrylate) acrylic acid (PMMA-AAc) patterning on glass substrate and albumin deposition onto colloid patterns by electrostatic interactions. We demonstrate that the colloid micropattern greatly improves the fluorescence imaging contrast for the deposited albumin due to its larger surface area and its immobilisation compared with patterning of albumin on a plane glass surface. This technique can be used to enhance the fluorescence imaging of biomarkers and other assays.

Graphical abstract: Fabrication of albumin-micropatterned surfaces by colloidal microcontact printing technique

Back to tab navigation

Publication details

The article was received on 29 Jan 2013, accepted on 07 May 2013 and first published on 07 May 2013


Article type: Paper
DOI: 10.1039/C3RA40507A
Citation: RSC Adv., 2013,3, 10420-10426
  •   Request permissions

    Fabrication of albumin-micropatterned surfaces by colloidal microcontact printing technique

    M. Ozmen, B. Ertekin, M. Ersoz and V. N. Paunov, RSC Adv., 2013, 3, 10420
    DOI: 10.1039/C3RA40507A

Search articles by author

Spotlight

Advertisements