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We report a facile method to design NIR ratiometric fluorescent probes in terms of analyte-induced carbamate cleavage. Two examples, CyNB and CyNN3, exhibit a significant analyte-triggered response with ratiometric fluorescence change in the NIR range and dual-emission ratiometry in living cells.

Graphical abstract: Development of ratiometric near-infrared fluorescent probes using analyte-specific cleavage of carbamate

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