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Abstract | Cited by

3-Cyanovinylcarbazole nucleoside (CNVK), which can quickly photocrosslink to the pyrimidine base in the DNA double strand, was incorporated into a triplex forming oligonucleotide (TFO). We successfully demonstrated that CNVK in TFO can photocrosslink to the thymine base in Watson–Crick double-stranded DNA via anti-Hoogsteen base pairing with a few seconds of photoirradiation.

Graphical abstract: Quick and reversible photocrosslinking reaction of 3-cyanovinylcarbazole nucleoside in a DNA triplex

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