Jump to main content
Jump to site search

Issue 7, 2013
Previous Article Next Article

Electrophoretically deposited reduced graphene oxide platform for food toxin detection

Author affiliations

Abstract

Reduced graphene oxide (RGO) due to its excellent electrochemical properties and large surface area, has recently aroused much interest for electrochemical biosensing application. Here, the chemically active RGO has been synthesized and deposited onto an indium tin oxide (ITO) coated glass substrate by the electrophoretic deposition technique. This novel platform has been utilized for covalent attachment of the monoclonal antibodies of aflatoxin B1 (anti-AFB1) for food toxin (AFB1) detection. The electron microscopy, X-ray diffraction, and UV-visible studies reveal successful synthesis of reduced graphene oxide while the XPS and FTIR studies suggest its carboxylic functionalized nature. The electrochemical sensing results of the anti-AFB1/RGO/ITO based immunoelectrode obtained as a function of aflatoxin concentration show high sensitivity (68 μA ng−1 mL cm−2) and improved detection limit (0.12 ng mL−1). The association constant (ka) for antigen–antibody interaction obtained as 5 × 10−4 ng mL−1 indicates high affinity of antibodies toward the antigen (AFB1).

Graphical abstract: Electrophoretically deposited reduced graphene oxide platform for food toxin detection

Back to tab navigation

Supplementary files

Publication details

The article was received on 11 Aug 2012, accepted on 14 Jan 2013 and first published on 17 Jan 2013


Article type: Paper
DOI: 10.1039/C3NR32242D
Nanoscale, 2013,5, 3043-3051

  •   Request permissions

    Electrophoretically deposited reduced graphene oxide platform for food toxin detection

    S. Srivastava, V. Kumar, M. A. Ali, P. R. Solanki, A. Srivastava, G. Sumana, P. S. Saxena, A. G. Joshi and B. D. Malhotra, Nanoscale, 2013, 5, 3043
    DOI: 10.1039/C3NR32242D

Search articles by author

Spotlight

Advertisements