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Issue 4, 2014
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Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

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Abstract

Soft micellar nanoparticles can be prepared from DNA conjugates designed to assemble via base pairing such that strands containing a polymer corona and a cholesterol tail generate controlled supramolecular architecture. Functionalization of one DNA conjugate strand with a biorecognition ligand results in shielding of the ligand when in the micelle, while encoding of the DNA sequences with overhangs allows supramolecular unpacking by addition of a complementary strand and sequence-specific unshielding of the ligand. The molecular assembly/disassembly and ‘on–off’ switch of the recognition signal is visualized by FRET pair signalling, PAGE and a facile turbidimetric binding assay, allowing direct and amplified readout of nucleic acid sequence recognition.

Graphical abstract: Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

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Supplementary files

Article information


Submitted
16 Sep 2013
Accepted
04 Nov 2013
First published
05 Nov 2013

This article is Open Access

Nanoscale, 2014,6, 2368-2374
Article type
Paper

Programmed assembly of polymer–DNA conjugate nanoparticles with optical readout and sequence-specific activation of biorecognition

J. P. Magnusson, F. Fernández-Trillo, G. Sicilia, S. G. Spain and C. Alexander, Nanoscale, 2014, 6, 2368
DOI: 10.1039/C3NR04952C

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