Issue 5, 2013
From the journal:

Nanoscale

Cellular uptake and trafficking of polydiacetylene micelles†

Edmond Gravel,a   Benoit Thézé,b   Isabelle Jacques,b   Parambath Anilkumar,a   Karine Gombert,b   Frédéric Ducongé*b  and  Eric Doris*a  

* Corresponding authors

a CEA, iBiTecS, Service de Chimie Bioorganique et de Marquage, 91191 Gif-sur-Yvette, France
E-mail: eric.doris@cea.fr
Fax: +33 169 08 79 91
Tel: +33 169 08 80 71

b CEA, I2BM, Service Hospitalier Frédéric Joliot and INSERM U1023, Laboratoire d'Imagerie Moléculaire Expérimentale, Université Paris Sud, 4 place du général Leclerc, 91401 Orsay, France
E-mail: frederic.duconge@cea.fr
Fax: +33 169 86 77 86
Tel: +33 169 86 77 66

Abstract

Polydiacetylene (PDA) micelles coated with either carboxylate-, ammonium-, or methoxy-polyethyleneglycol (PEG) chains were assembled and loaded with a fluorescent dye (DiO). Their interaction with MCF-7 human breast tumor cells was investigated by epi-fluorescence microscopy and fluorescence-activated cell sorting (FACS) to determine their internalization pathway and intracellular fate. It was found that the ionic character of the micelles influenced their internalization kinetics through a caveolae-mediated pathway and that all micelle types behaved somewhat similarly inside cells.

Graphical abstract: Cellular uptake and trafficking of polydiacetylene micelles

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Article information

DOI
https://doi.org/10.1039/C2NR34149B
Article type
Paper
Submitted
17 Dec 2012
Accepted
25 Dec 2012
First published
04 Jan 2013

Nanoscale, 2013,5, 1955-1960

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Cellular uptake and trafficking of polydiacetylene micelles

E. Gravel, B. Thézé, I. Jacques, P. Anilkumar, K. Gombert, F. Ducongé and E. Doris, Nanoscale, 2013, 5, 1955 DOI: 10.1039/C2NR34149B

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