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Issue 6, 2013
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Direct in vivo imaging of essential bioinorganics in Caenorhabditis elegans

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Abstract

Metals are essential in biochemistry, with highly regulated localisation and concentration. To continue elucidating the impact of inorganic physiology on cellular process in health and disease requires development of suitable eukaryotic models and experimental approaches. The multicellular organism Caenorhabditis elegans has been widely adopted as a model system and proved to be highly valuable in biomedical research, and here we record the distribution of physiologically important 4th row elements throughout anesthetised, hydrated adult C. elegans without the need for chemical fixation or mechanical sectioning. Probing the partitioning of elements between intact anatomical structures at subcellular length scales allowed the impact of preparation techniques commonly in use for analytical microanalysis to be assessed. In addition, taking advantage of C. elegans as a research tool, these studies were carried out across a cohort of genetically homogenous individuals with specific expression of green fluorescent protein within intestinal cells. These studies allowed direct visualisation of elemental co-localisation with anatomical structures and facilitated analysis of inter-animal variability in biometal abundance and localisation. These data reflect stochastic metabolic activity and confirm C. elegans as a sensitive system in which to explore the effects of altered metal homeostasis.

Graphical abstract: Direct in vivo imaging of essential bioinorganics in Caenorhabditis elegans

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Publication details

The article was received on 09 Jan 2013, accepted on 20 Feb 2013 and first published on 22 Feb 2013


Article type: Paper
DOI: 10.1039/C3MT00010A
Citation: Metallomics, 2013,5, 627-635
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    Direct in vivo imaging of essential bioinorganics in Caenorhabditis elegans

    S. A. James, M. D. de Jonge, D. L. Howard, A. I. Bush, D. Paterson and G. McColl, Metallomics, 2013, 5, 627
    DOI: 10.1039/C3MT00010A

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