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Issue 23, 2013
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Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA

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Abstract

LATE-PCR is an advanced form of non-symmetric PCR that efficiently generates single-stranded DNA which can readily be characterized at the end of amplification by hybridization to low-temperature fluorescent probes. We demonstrate here for the first time that monoplex and duplex LATE-PCR amplification and probe target hybridization can be carried out in double layered PDMS microfluidics chips containing dried reagents. Addition of a set of reagents during dry down overcomes the common problem of single-stranded oligonucleotide binding to PDMS. These proof-of-principle results open the way to construction of inexpensive point-of-care devices that take full advantage of the analytical power of assays built using LATE-PCR and low-temperature probes.

Graphical abstract: Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA

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Publication details

The article was received on 16 Sep 2013, accepted on 25 Sep 2013 and first published on 25 Sep 2013


Article type: Paper
DOI: 10.1039/C3LC51049B
Lab Chip, 2013,13, 4635-4641

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    Construction of a microfluidic chip, using dried-down reagents, for LATE-PCR amplification and detection of single-stranded DNA

    Y. Jia, P. Mak, C. Massey, R. P. Martins and L. J. Wangh, Lab Chip, 2013, 13, 4635
    DOI: 10.1039/C3LC51049B

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