Jump to main content
Jump to site search
Access to RSC content Close the message box

Continue to access RSC content when you are not at your institution. Follow our step-by-step guide.


Issue 24, 2013
Previous Article Next Article

Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

Author affiliations

Abstract

Here, we present a platform to detect cytokine (IL-2, IFN-γ, TNF-α) secretion of single, activated T-cells in droplets over time. We use a novel droplet-based microfluidic approach to encapsulate cells in monodisperse agarose droplets together with functionalized cytokine-capture beads for subsequent binding and detection of secreted cytokines from single cells. This method allows high-throughput detection of cellular heterogeneity and maps subsets within cell populations with specific functions.

Graphical abstract: Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

Back to tab navigation

Supplementary files

Article information


Submitted
13 Aug 2013
Accepted
22 Oct 2013
First published
22 Oct 2013

Lab Chip, 2013,13, 4740-4744
Article type
Communication

Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

V. Chokkalingam, J. Tel, F. Wimmers, X. Liu, S. Semenov, J. Thiele, C. G. Figdor and W. T. S. Huck, Lab Chip, 2013, 13, 4740
DOI: 10.1039/C3LC50945A

Social activity

Search articles by author

Spotlight

Advertisements