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Issue 24, 2013
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Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

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Abstract

Here, we present a platform to detect cytokine (IL-2, IFN-γ, TNF-α) secretion of single, activated T-cells in droplets over time. We use a novel droplet-based microfluidic approach to encapsulate cells in monodisperse agarose droplets together with functionalized cytokine-capture beads for subsequent binding and detection of secreted cytokines from single cells. This method allows high-throughput detection of cellular heterogeneity and maps subsets within cell populations with specific functions.

Graphical abstract: Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

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Article information


Submitted
13 Aug 2013
Accepted
22 Oct 2013
First published
22 Oct 2013

Lab Chip, 2013,13, 4740-4744
Article type
Communication

Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics

V. Chokkalingam, J. Tel, F. Wimmers, X. Liu, S. Semenov, J. Thiele, C. G. Figdor and W. T. S. Huck, Lab Chip, 2013, 13, 4740
DOI: 10.1039/C3LC50945A

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