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Issue 22, 2013
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Parallel measurements of reaction kinetics using ultralow-volumes

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Abstract

We present a new platform for the production and manipulation of microfluidic droplets in view of measuring the evolution of a chemical reaction. Contrary to existing approaches, our device uses gradients of confinement to produce a single drop on demand and guide it to a pre-determined location. In this way, two nanoliter drops containing different reagents can be placed in contact and merged together, in order to trigger a chemical reaction. The reaction rate is extracted from an analysis of the observed reaction–diffusion front. We show that the results obtained using this platform are in excellent agreement with stopped-flow measurements, while decreasing the sample consumption 5000 fold. We also show how the device operation can be parallelized in order to react an initial sample with a range of compounds or concentrations, on a single integrated chip. This integrated chip thus further reduces sample consumption while reducing the time required for the experimental runs from hours to minutes.

Graphical abstract: Parallel measurements of reaction kinetics using ultralow-volumes

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Article information


Submitted
26 Jun 2013
Accepted
28 Aug 2013
First published
29 Aug 2013

Lab Chip, 2013,13, 4326-4330
Article type
Paper

Parallel measurements of reaction kinetics using ultralow-volumes

E. Fradet, P. Abbyad, M. H. Vos and C. N. Baroud, Lab Chip, 2013, 13, 4326
DOI: 10.1039/C3LC50768H

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