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Issue 23, 2013
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Microfluidic-based patterning of embryonic stem cells for in vitro development studies

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Abstract

In vitro recapitulation of mammalian embryogenesis and examination of the emerging behaviours of embryonic structures require both the means to engineer complexity and accurately assess phenotypes of multicellular aggregates. Current approaches to study multicellular populations in 3D configurations are limited by the inability to create complex (i.e. spatially heterogeneous) environments in a reproducible manner with high fidelity thus impeding the ability to engineer microenvironments and combinations of cells with similar complexity to that found during morphogenic processes such as development, remodelling and wound healing. Here, we develop a multicellular embryoid body (EB) fusion technique as a higher-throughput in vitro tool, compared to a manual assembly, to generate developmentally relevant embryonic patterns. We describe the physical principles of the EB fusion microfluidic device design; we demonstrate that >60 conjoined EBs can be generated overnight and emulate a development process analogous to mouse gastrulation during early embryogenesis. Using temporal delivery of bone morphogenic protein 4 (BMP4) to embryoid bodies, we recapitulate embryonic day 6.5 (E6.5) during mouse embryo development with induced mesoderm differentiation in murine embryonic stem cells leading to expression of Brachyury-T–green fluorescent protein (T–GFP), an indicator of primitive streak development and mesoderm differentiation during gastrulation. The proposed microfluidic approach could be used to manipulate hundreds or more of individual embryonic cell aggregates in a rapid fashion, thereby allowing controlled differentiation patterns in fused multicellular assemblies to generate complex yet spatially controlled microenvironments.

Graphical abstract: Microfluidic-based patterning of embryonic stem cells for in vitro development studies

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Article information


Submitted
30 May 2013
Accepted
14 Sep 2013
First published
17 Sep 2013

Lab Chip, 2013,13, 4617-4624
Article type
Paper

Microfluidic-based patterning of embryonic stem cells for in vitro development studies

S. Suri, A. Singh, A. H. Nguyen, A. M. Bratt-Leal, T. C. McDevitt and H. Lu, Lab Chip, 2013, 13, 4617
DOI: 10.1039/C3LC50663K

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