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Issue 22, 2013
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μ-eLCR: a microfabricated device for electrochemical detection of DNA base changes in breast cancer cell lines

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Abstract

Microfabricated devices for the electrochemical detection of single DNA base changes in cancer cell lines are highly desirable due to the inherent advantages such as portability, simplicity, and the rapid and inexpensive nature of electrochemical readout methods. Moreover, molecular sensors that use microscale-footprint working electrodes have shown high signal-to-noise ratio. Herein we report a microdevice-based electrochemical assay (μ-eLCR) measuring ligase chain reaction (LCR)-amplified long and short “knife” motifs which reflect the presence or absence of a DNA base change of interest in a target sequence. This μ-eLCR approach has higher sensitivity (4.4 to 10 fold improvement over macrodisk electrodes) and good reproducibility (%RSD 6.5%, n = 12) for the detection of LCR-amplified DNA bases. The devices also exhibited excellent sensitivity for the detection of DNA methylation (C to T base change in a locus associated with cancer metastasis) in two cell lines and serum derived DNA samples. We believe that the μ-eLCR device may be a useful diagnostic tool for inexpensive and rapid detection of single DNA base change applications such as DNA methylation and single nucleotide polymorphism (SNP) detection.

Graphical abstract: μ-eLCR: a microfabricated device for electrochemical detection of DNA base changes in breast cancer cell lines

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Supplementary files

Article information


Submitted
29 Apr 2013
Accepted
23 Aug 2013
First published
23 Aug 2013

Lab Chip, 2013,13, 4385-4391
Article type
Paper

μ-eLCR: a microfabricated device for electrochemical detection of DNA base changes in breast cancer cell lines

E. J. H. Wee, S. Rauf, K. M. Koo, M. J. A. Shiddiky and M. Trau, Lab Chip, 2013, 13, 4385
DOI: 10.1039/C3LC50528F

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