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Issue 20, 2013
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Micro flow reactor chips with integrated luminescent chemosensors for spatially resolved on-line chemical reaction monitoring

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Abstract

Real-time chemical reaction monitoring in microfluidic environments is demonstrated using luminescent chemical sensors integrated in PDMS/glass-based microscale reactors. A fabrication procedure is presented that allows for straightforward integration of thin polymer layers with optical sensing functionality in microchannels of glass–PDMS chips of only 150 μm width and of 10 to 35 μm height. Sensor layers consisting of polystyrene and an oxygen-sensitive platinum porphyrin probe with film thicknesses of about 0.5 to 4 μm were generated by combining spin coating and abrasion techniques. Optimal coating procedures were developed and evaluated. The chip-integrated sensor layers were calibrated and investigated with respect to stability, reproducibility and response times. These microchips allowed observation of dissolved oxygen concentration in the range of 0 to over 40 mg L−1 with a detection limit of 368 μg L−1. The sensor layers were then used for observation of a model reaction, the oxidation of sulphite to sulphate in a microfluidic chemical reactor and could observe sulphite concentrations of less than 200 μM. Real-time on-line monitoring of this chemical reaction was realized at a fluorescence microscope setup with 405 nm LED excitation and CCD camera detection.

Graphical abstract: Micro flow reactor chips with integrated luminescent chemosensors for spatially resolved on-line chemical reaction monitoring

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Article information


Submitted
26 Mar 2013
Accepted
30 Jul 2013
First published
31 Jul 2013

Lab Chip, 2013,13, 4134-4141
Article type
Paper

Micro flow reactor chips with integrated luminescent chemosensors for spatially resolved on-line chemical reaction monitoring

L. Gitlin, C. Hoera, R. J. Meier, S. Nagl and D. Belder, Lab Chip, 2013, 13, 4134
DOI: 10.1039/C3LC50387A

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