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Issue 18, 2013
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Microfluidic titer plate for stratified 3D cell culture

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Abstract

Human tissues and organs are inherently heterogeneous. Their functionality is determined by the interplay between different cell types, their secondary architecture, vascular system and gradients of signaling molecules and metabolites. Here we propose a stratified 3D cell culture platform, in which adjacent lanes of gels and liquids are patterned by phaseguides to capture this tissue heterogeneity. We demonstrate 3D cell culture of HepG2 hepatocytes under continuous perfusion, a rifampicin toxicity assay and co-culture with fibroblasts. 4T1 breast cancer cells are used to demonstrate invasion and aggregation models. The platform is incorporated in a microtiter plate format that renders it fully compatible with automation and high-content screening equipment. The extended functionality, ease of handling and full compatibility to standard equipment is an important step towards adoption of Organ-on-a-Chip technology for screening in an industrial setting.

Graphical abstract: Microfluidic titer plate for stratified 3D cell culture

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Supplementary files

Article information


Submitted
15 Feb 2013
Accepted
25 Jun 2013
First published
26 Jul 2013

Lab Chip, 2013,13, 3548-3554
Article type
Paper

Microfluidic titer plate for stratified 3D cell culture

S. J. Trietsch, G. D. Israëls, J. Joore, T. Hankemeier and P. Vulto, Lab Chip, 2013, 13, 3548
DOI: 10.1039/C3LC50210D

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