Issue 8, 2013

Measurement and modeling of acid dissociation constants of tri-peptides containing Glu, Gly, and His using potentiometry and generalized multiplicative analysis of variance

Abstract

We report pKa values with measurement uncertainties for all labile protons of the 27 tri-peptides prepared from the amino acids glutamic acid (E), glycine (G) and histidine (H). Each tri-peptide (GGG, GGE, GGH, …, HHH) was subjected to alkali titration and pKa values were calculated from triplicate potentiometric titrations data using HyperQuad 2008 software. A generalized multiplicative analysis of variance (GEMANOVA) of pKa values for the most acidic proton gave the optimum model having two terms, an interaction between the end amino acids plus an isolated main effect of the central amino acid.

Graphical abstract: Measurement and modeling of acid dissociation constants of tri-peptides containing Glu, Gly, and His using potentiometry and generalized multiplicative analysis of variance

Supplementary files

Article information

Article type
Paper
Submitted
18 Sep 2012
Accepted
04 Dec 2012
First published
05 Dec 2012

Dalton Trans., 2013,42, 2940-2947

Measurement and modeling of acid dissociation constants of tri-peptides containing Glu, Gly, and His using potentiometry and generalized multiplicative analysis of variance

R. R. Khoury, G. J. Sutton, D. B. Hibbert and D. Ebrahimi, Dalton Trans., 2013, 42, 2940 DOI: 10.1039/C2DT32797J

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