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Issue 100, 2013
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High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway

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Abstract

We report a high-throughput assay for H2S-producing enzymes, which is based on a newly designed tandem-well plate. Screening of 21 599 agents identified several potent inhibitors of cystathionine β-synthase and cystathionine γ-lyase, the two key enzymes generating H2S in mammals, with IC50 values in the low two-digit micromolar range.

Graphical abstract: High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway

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Submitted
03 Sep 2013
Accepted
24 Oct 2013
First published
24 Oct 2013

Chem. Commun., 2013,49, 11782-11784
Article type
Communication

High-throughput tandem-microwell assay identifies inhibitors of the hydrogen sulfide signaling pathway

Y. Zhou, J. Yu, X. Lei, J. Wu, Q. Niu, Y. Zhang, H. Liu, P. Christen, H. Gehring and F. Wu, Chem. Commun., 2013, 49, 11782
DOI: 10.1039/C3CC46719H

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